FastQCFastQC Report
Sun 14 Apr 2019
SRR6575539_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575539_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017433
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA81370.7997578218909747No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC62350.6128167653300021No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC54330.5339909360124941No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC28550.28060815798190153No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG22650.2226190815513159No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG20870.20512407205191888No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC19600.1926416776338098No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT19040.18713762970141523No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA16900.1661043036740503No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT16540.16256598714608234No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT16480.16197626772475435No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC16300.16020710946077038No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT16040.15765165863501576No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA16000.15725851235413044No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA15880.1560790735114745No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA15680.15411334210704783No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT15670.15401505553682648No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA15500.15234418384306386No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT14970.1471349956213333No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT14800.14546412392757066No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG13210.12983655926237897No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT12800.12580680988330434No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT12030.11823874397626183No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA11810.11607643943139254No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA11470.11273469604386727No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA11370.11175183034165394No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG10900.10713236154125136No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG10850.1066409286901447No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA10570.10388890472394742No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC10220.10044887476620082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3800.054.432841
GTATCAA19850.050.2073441
TCAACGC20500.047.21864
ATCAACG20600.046.9893843
TATCAAC21600.045.271292
CAACGCA21550.044.9157185
AACGCAG21700.044.605246
ACGCAGA26350.036.7337277
AGGTATA16600.033.6835066
CTTAGGT16850.033.464253
TAGGTAT16950.033.2651865
ACCTAAG28300.033.222741
ACCAGAT28750.032.85170794
TTAGGTA17200.032.783294
GTCTTAG17800.032.4845621
TAAGACA31050.032.3855974
GGTATAG17400.031.8647987
TCTTAGG18000.031.8640212
CTAAGAC29400.031.8057423
CCTAAGA29850.031.656652
Produced by FastQC (version 0.11.2)