FastQCFastQC Report
Sun 14 Apr 2019
SRR6575546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559051
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19050.34075603120287773No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC16200.28977678244024246No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT13530.24201727570472104No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG9790.17511819136357862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9600.17171957477940294No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC9140.16349134515455657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.13809115805176986No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7650.13683903615233672No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA7630.13648128703821297No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT7490.13397704323934667No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT7430.1329037958969754No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7040.12592768817156216No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA6880.12306569525857211No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT6340.1134064691772307No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT6220.11125997449248817No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG6100.10911347980774562No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC6050.10821910702243623No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC5960.10660923600887932No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA5760.10303174486764176No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA5760.10303174486764176No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA5670.10142187385408485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23750.050.8871771
GTACTAG3700.049.5680431
GGTATCA8450.049.5304341
TAGTATG503.0086956E-546.9966477
TAATAGT601.604436E-646.9966434
TAGGCAT5100.042.3891335
TCTAGAC704.6332407E-640.282843
CAACGCA30300.039.086325
ATCAACG30200.039.0601273
CTAGGCA5700.038.751624
GTGCAAG2250.037.620871
CTTAGGT651.4131542E-436.1512683
AACGCAG34650.035.94266
TCAACGC33250.035.618514
GTTAATA1601.8189894E-1235.2695661
GTATATA801.15319235E-535.2695661
AAGGGTA2050.034.3877875
GTACATG38200.034.1000671
TATCAAC35600.033.948612
ACATGGG39750.032.9863243
Produced by FastQC (version 0.11.2)