FastQCFastQC Report
Sun 14 Apr 2019
SRR6575547_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575547_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532923
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18700.35089496981740326No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14340.2690820249829713No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT12150.22798790819686895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.1891455238374024No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG9040.16963050947322594No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC8760.16437646714440923No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA7570.14204678724693812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7120.13360279064705408No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7050.13228928006484988No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7050.13228928006484988No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT6760.12684759336714685No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA6750.12665994899826055No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT6610.12403292783385218No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT6260.11746537492283125No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA6200.11633950870951339No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT5800.10883373395406092No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA5760.10808315647851566No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5410.10151560356749476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23800.057.2778851
GGTATCA6900.053.1387671
ATCAACG28850.045.935343
CAACGCA29600.044.608495
TATCAAC32200.042.0478022
GTACTAG2500.041.3664861
AACGCAG33300.040.7808726
TCAACGC32900.040.1378484
ACGCAGA35750.036.934587
CGCAGAG36450.036.2252738
CTAACTG1204.2036845E-935.24564
GCAGAGT38400.034.2601289
TAGTACT4200.033.5672384
ACCGTCC702.1862409E-433.5640878
ATACGGG2550.033.1723333
GGCGTCG2550.033.1692168
AGGTATA1001.4116922E-632.8928076
GGTAATC2300.032.6885038
TACGGGC2600.032.53444
TAGGCAT4400.032.038455
Produced by FastQC (version 0.11.2)