FastQCFastQC Report
Sun 14 Apr 2019
SRR6575548_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575548_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159607
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2860.17919013577098747No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.15600819512928632No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1770.11089739171840833No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1730.10839123597335956No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC200.002159987370.4964753
ACGCCTA250.005228840356.3971826
GTATCAA5050.047.582231
ATCAACG6100.039.293123
CGACTAT250.001700603937.5981246-47
GGCTCGA250.001700603937.5981254-55
GTACATG11750.037.2916031
TACATGG11750.037.1981432
TCAACGC6450.037.1609344
CAACGCA6650.036.0433125
AACGCAG6650.036.0433126
CATGGGT3150.035.807734
ACATGGG12250.035.6798483
CATGGGG4250.034.280644
ACGGTAT550.002710700534.180119
TATCAAC7150.033.52282
ATAGACT1001.4016459E-632.8983574
ATGGGGC1052.0514744E-631.3317665
CGCAGAG7800.030.7292358
ATGGGGG1901.2732926E-1129.6827265
Produced by FastQC (version 0.11.2)