FastQCFastQC Report
Sun 14 Apr 2019
SRR6575559_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575559_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148333
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2520.16988802222027466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.13011265193854368No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1580.10651709329683888No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1510.1017979815684979No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA156.898225E-493.97274
CCCGCTA156.898225E-493.97273
TACATGG12250.040.3011862
ACATGGG12150.039.8320473
GTACATG12750.039.8270531
CATGGGG4100.037.818284
TGCCCGC500.001694050537.614441
CATGGGT2350.035.989544
GTCATAA550.002704318634.1949421
ATGGGAG2450.032.602775
CTATACA753.2674722E-431.3242324
CATGGGA6850.030.181014
GTATCAA5850.028.9341851
ATGGGGG1802.3464963E-1028.713885
ATGGGTA1154.1735166E-628.6003845
CTAGACA1005.30326E-528.1918074
TTTATAC1005.30326E-528.1918073
ATGGGAC1205.8114383E-627.4087035
TATACAG1057.393427E-526.8493425
CTATTCT700.00882417326.849344
Produced by FastQC (version 0.11.2)