FastQCFastQC Report
Sun 14 Apr 2019
SRR6575559_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575559_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148333
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG2670.180000404495291No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.17056218103860907No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2090.14089919303189447No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1790.12067442848186175No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1620.10921372857017655No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1620.10921372857017655No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1540.10382045802350118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAA405.5813574E-447.1345941
TAGCCCT902.4920155E-1046.9915734
ATAGCCC450.001011990741.7702873
GTATCAA6000.040.8499831
GGGTACC608.7962355E-539.159647
TCTTCCG500.001693125137.6186294
ATGGGAC1251.4915713E-1037.5932545
AACGCAG7250.033.70436
ATCAACG7650.032.556253
ACATGGG11800.032.2569273
GTACATG12100.031.9424531
CAACGCA7800.031.930175
TCAACGC7700.031.7345684
TACATGG11950.031.4587922
ATGGGTA1052.0522475E-631.3277155
ATGGGAG2400.031.3277135
TAATACC600.004153056531.3277134
TACCCTG600.004153056531.3277135
TATGGGC600.004153056531.3277133
ACGCAGA8200.029.7995327