FastQCFastQC Report
Sun 14 Apr 2019
SRR6575562_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575562_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140579
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA77090.6758847918469479No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC54120.4744958481613286No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC41080.36016794978690647No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC22380.19621613233278887No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG17740.15553503965968163No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG17160.1504499030755432No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT15910.13949055698903803No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT15630.13703566346566087No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT15090.13230122595629062No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC13940.12221862755670584No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT13890.12178025371324563No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA12980.11380184976226987No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT12840.11257440300058129No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG12580.11029485901458821No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA12480.10941811132766778No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG12240.1073139168790588No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA11680.10240412983230447No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT11480.10065063445846363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5650.063.4017641
GTATCAA19550.051.835571
ATCAACG23550.042.511323
TCAACGC23450.042.4903074
ACCTAAG23700.040.5712171
CAACGCA24700.040.339995
AGGTATA14950.039.926336
CTTAGGT14850.039.8804443
AACGCAG25400.039.5983356
TATCAAC25050.039.5904662
TTAGGTA14900.039.429444
TAGGTAT15000.039.1665765
GTCTTAG16150.038.8164061
TAAGACA26300.038.4219634
CTAAGAC25700.038.2233733
CCTAAGA26550.036.822622
GGTATAG16300.036.0428627
GTATAGT16700.035.4594428
TCTTAGG17600.035.2514652
TATAGTA17100.034.629989
Produced by FastQC (version 0.11.2)