FastQCFastQC Report
Sun 14 Apr 2019
SRR6575563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073418
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA75660.7048512322319916No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC58050.5407958502652275No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC42180.39295036975344183No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC25600.2384905041651994No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG20910.19479829851930935No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG18510.17243981375382192No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT16370.15250349817126227No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT15500.14439854744377306No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA15200.14160373684808714No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC14820.13806364342688496No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT14710.1370388795418001No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT14370.13387142753335607No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT13730.12790916492922608No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC13280.12371694903569719No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA12910.12027001596768454No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT12850.11971105384854736No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT12660.11794100713794625No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT12250.11412143265717549No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA12080.11253770665295346No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA12050.11225822559338487No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG11980.11160610312105815No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA11630.10834549075942457No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA10890.10145162462339927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5100.061.7583241
GTATCAA20700.049.2810861
ATCAACG21900.044.6280333
TCAACGC22350.043.729484
CAACGCA23300.041.946525
AACGCAG23800.041.262726
TTAGGTA15550.041.095764
GTCTTAG15750.040.8912961
CTTAGGT15750.039.9772343
TATCAAC24700.039.968072
TAGGTAT15800.039.8507235
AGGTATA15900.039.600096
GGTATAG16800.037.4786577
TCTTAGG17350.037.1203422
AGGGTAA4850.035.846646
GTATAGT17600.035.775088
GGTAATC4750.035.6120878
TATAGTA17850.035.272399
ACCTAAG25600.034.1557541
ACGCAGA29500.033.1306427
Produced by FastQC (version 0.11.2)