FastQCFastQC Report
Sun 14 Apr 2019
SRR6575563_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575563_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073418
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA78560.7318677346569556No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC54300.5058607178191534No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC42510.39602466140869635No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC21910.20411433383826244No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG17720.16508014585184896No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG17200.16023580748599334No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT15690.14616859415437417No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT15290.1424421800267929No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT14970.13946104872472792No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT14390.13405774823973512No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC14140.13172873940999685No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT13620.12688440104414123No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG13400.12483487327397155No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA13070.12176058161871703No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA12380.11533251724863938No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG12350.1150530361890708No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA12280.11440091371674409No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT11980.11160610312105815No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT11640.10843865111261408No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA11530.10741388722752927No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA11530.10741388722752927No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA11420.10638912334244442No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC11070.10312851098081084No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA11020.10266270921486319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4600.068.566911
GTATCAA17950.053.763431
ATCAACG20900.044.5212363
TCAACGC21600.043.295994
CAACGCA22300.041.9369245
AACGCAG22500.041.7730146
TATCAAC24700.038.2426032
TTAGGTA14100.036.329194
TAGGTAT14250.035.6169935
AGGTATA14650.034.6445126
GTCTTAG15500.034.623481
CTTAGGT14500.034.0306053
ACGCAGA27600.033.883827
ACCTAAG25200.032.8783261
CTAAGAC26450.032.514253
TAAGACA27100.032.2546234
TCTTAGG16550.032.0869752
GTATAGT15600.031.9530968
TATAGTA15950.031.8415939
GGTATAG16100.031.2324627
Produced by FastQC (version 0.11.2)