FastQCFastQC Report
Sun 14 Apr 2019
SRR6575573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232837
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA88290.7161530680860487No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC54530.4423131362864677No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC33510.2718120887027239No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG29980.24317894417510183No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC28620.23214747772819927No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC23680.19207729813430324No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA21230.1722044357851038No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA20250.164255290845424No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC18410.14933036565255586No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT16060.13026864054209922No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT15630.12678075041550504No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT15310.12418511125152798No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC15040.12199504070692233No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT14840.12037276622943666No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA14440.11712821727446533No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC14210.1152626016253568No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA14060.11404589576724256No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA14010.11364032714787113No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA13540.10982798212577981No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG13070.10601563710368848No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT12470.10114881367123146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10250.051.3734551
GTATCAA39350.049.1068041
ATCAACG41050.045.3385933
TATCAAC41400.045.312412
CAACGCA41700.044.7445875
AGGGTAA7050.044.665456
TCAACGC43100.043.073074
AACGCAG44800.041.858246
AAGGGTA8050.041.4522935
GGTAATC7850.039.514858
ATAAGCG608.825869E-539.16563
TTAGGTA14300.039.1108254
GTGCAAG9400.038.5130121
TAGGTAT14900.037.5358925
CTTAGGT14650.037.5348133
AGGTATA15300.036.554566
ACGCAGA52200.035.6542057
GTCTTAG16600.035.12031
GGTATAG16050.034.8464057
CGCAGAG54750.033.9935958
Produced by FastQC (version 0.11.2)