FastQCFastQC Report
Sun 14 Apr 2019
SRR6575573_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575573_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232837
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA92290.7485985576357621No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC53450.4335528541080451No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC31770.25769830074859856No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC25930.21032788600601704No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG25470.20659665470779998No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC22240.1803969218964064No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT17790.14430131477235028No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG17710.143652404981356No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA16600.13464878163131053No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA16480.13367541694481916No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC16210.13148534640021348No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT15020.12183281325917376No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT14770.11980497016231667No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC14510.11769601334158532No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG14330.1162359663118482No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA14240.11550594279697965No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC13730.11136914287939118No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA13650.11072023308839692No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC13640.11063911936452263No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13590.11023355074515123No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA12990.10536672731269421No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA12730.10325777049196286No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT12580.1020410646338486No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA12500.10139215484285434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10750.054.8338471
GTATCAA36150.049.8313031
GTACTAG3750.046.528351
TATCAAC39600.044.396342
ATCAACG40200.043.499843
CAACGCA40400.043.1646355
TCAACGC41850.042.0077364
AACGCAG43200.040.6933446
TTAGGTA15450.038.943474
AGGTATA15450.038.941896
TAGGTAT15850.038.552245
CTTAGGT15650.038.1469763
GGTATAG16200.037.1329967
AAGGGTA5600.036.092425
ACCTAAG27300.035.9292261
GGTAATC5700.035.4534658
GTCTTAG17750.035.334621
GTATAGT18050.034.108238
CTAAGAC28900.033.9953273
AGGGTAA6000.033.6862536
Produced by FastQC (version 0.11.2)