FastQCFastQC Report
Sun 14 Apr 2019
SRR6575574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133548
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA85280.752328088444424No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC52160.46014813664705856No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC30460.2687138083257172No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC27370.24145426572143394No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG27300.2408367356300748No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA20790.18340643713367233No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC20290.1789955079096783No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA19630.17317308133400613No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC18310.16152822818266188No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT16010.14123795375228929No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC15120.13338649973357988No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT14410.12712298023550833No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT14170.1250057342079912No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT14070.12412354836319238No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA13490.11900687046335928No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA13350.11777181028064096No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA12830.11318444388768716No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG12440.10974391909297179No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA12350.10894995183265287No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC12020.10603873854481681No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11710.10330396242594049No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT11680.10303930667250087No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT11580.10215712082770204No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT11370.10030453055362455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.058.5371631
GTATCAA36400.052.2923851
TATCAAC38300.048.1051142
ATCAACG38050.047.5439263
CAACGCA38450.047.293745
TCAACGC39800.045.5714874
AACGCAG40650.045.080956
AGGGTAA6950.043.269736
AAGGGTA7200.041.1146975
ACGCAGA47450.038.3233767
GGTAATC7500.038.2170878
CGCAGAG48550.037.4550828
GGGTAAT8000.035.8285187
GCAGAGT52850.034.4950339
TTAGGTA12750.034.2737584
TAGGTAT12950.033.744445
AGGTATA12900.033.510986
GTCTTAG15100.033.3036351
CTTAGGT13550.032.2502173
ACCTAAG26000.031.6336651
Produced by FastQC (version 0.11.2)