FastQCFastQC Report
Sun 14 Apr 2019
SRR6575579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205596
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA87440.7252844236377692No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC56880.4717998400790978No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC34390.28525310302953893No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG29860.24767832673631965No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC25240.209357031708798No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA21500.17833503097223283No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC21210.1759295817172585No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA17300.1434974900381222No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC16540.13719355405956887No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT16370.13578346311699774No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT15960.13238265554962028No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT15140.12558104041486534No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA14120.11712049475943848No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC13880.11512977813463218No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT13860.11496388508256497No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG13720.11380263371809463No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA13270.11007004004658276No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA13030.10807932342177645No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT12900.1070010185833397No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT12650.10492735543249977No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA12460.10335137143786144No Hit
TCCAGGAACTGTGCAAAGTCATCCATGACAGTCTTCAGTTGCTCCGCTGT12180.10102886870892074No Hit
ATCCTGAACCGTGTGTGTCTGCTGCATGAGAAGACCCCAGTGAGTGAGCA12110.10044824302668555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6350.066.628611
GTATCAA28000.050.0322721
TATCAAC30500.045.1606062
ATCAACG30900.044.5649073
TCAACGC31350.043.925224
CAACGCA32000.043.032995
GGTAATC4900.042.202728
AACGCAG33300.041.494166
TTAGGTA15850.039.7337384
TAGGTAT15800.038.669645
CTTAGGT16250.038.4664543
AGGTATA16100.037.9490856
AAGGGTA5850.037.7594685
GTCTTAG18150.037.038331
AGGGTAA5700.036.2795336
GGTATAG17750.034.950997
ACCTAAG28500.034.3091851
GTACATG66750.033.1008071
TCTTAGG20500.033.0217862
ACGCAGA41900.032.9774557
Produced by FastQC (version 0.11.2)