FastQCFastQC Report
Sun 14 Apr 2019
SRR6575579_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575579_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205596
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA93900.7788678794554726No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC56560.4691455512460227No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC35190.29188882511222664No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG24330.20180889783974068No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC22830.18936691893470117No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC20780.17236288109781386No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT17500.14515642055879416No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA17090.1417556129914167No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT15510.12865006187810843No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC15310.1269911313574365No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT15250.1264934522012349No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC13990.11604218992100172No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG13940.11562745729083374No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA13640.11313906150982585No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG13550.11239254277552346No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA13430.11139718446312032No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA12740.10567387416680214No Hit
TCCAGGAACTGTGCAAAGTCATCCATGACAGTCTTCAGTTGCTCCGCTGT12530.10393199712009661No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT12430.10310253185976065No Hit
ATCCTGAACCGTGTGTGTCTGCTGCATGAGAAGACCCCAGTGAGTGAGCA12300.1020242270213239No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT12100.10036529650065196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6050.059.1877061
GTATCAA23600.054.3037951
TATCAAC29500.042.5383262
TAGGTAT16200.041.7771345
TTAGGTA16050.041.581914
ATCAACG30200.041.3967133
TCAACGC30450.040.5937924
CAACGCA30950.039.4824265
CTTAGGT17100.039.3034863
AGGTATA17150.038.6408046
AACGCAG32650.038.0024766
GTCTTAG19150.037.6440161
GGTATAG17800.037.49387
GTATAGT19300.036.5279358
TATAGTA19550.035.8189359
GGTAATC3350.035.0740858
ACCTAAG27800.033.557811
CTAAGAC30000.032.8994943
TCTTAGG21500.032.790192
GTACATG61350.031.6414151
Produced by FastQC (version 0.11.2)