FastQCFastQC Report
Sun 14 Apr 2019
SRR6575580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097731
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA81130.7390699542966355No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC53180.4844538416060037No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC33160.30207764926015573No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG27740.2527030757079831No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC24490.2230965509765143No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC20600.18765981829792544No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA19120.17417746242021043No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA17040.15522928659207036No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC16970.15459160759785412No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT15160.13810305074740534No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT14890.13564343176971408No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT14180.12917554482837781No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA14020.1277179928415978No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA13660.1244385008713428No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC13140.11970145691430778No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT12790.11651306194322654No Hit
TCCAGGAACTGTGCAAAGTCATCCATGACAGTCTTCAGTTGCTCCGCTGT12520.11405344296553527No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA12300.11204930898371278No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT12000.10931639900850025No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA11930.10867872001428401No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT11830.1077677500225465No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT11760.10713007102833025No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG11620.10585471303989775No Hit
TCCTTAGGGTGTTGATTTTACACCAACAGAAAAGATGAGTCCTGAGTCTT11280.10275741506799024No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT11090.101026572083689No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG11010.100297796090299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7200.062.666211
GTATCAA26700.048.231861
TATCAAC28500.043.701442
ATCAACG28750.042.982723
TCAACGC29450.041.961064
CAACGCA29750.041.537925
AACGCAG30800.040.7320756
AAGGGTA5200.039.758075
GGTAATC5050.039.0781448
AGGGTAA5600.036.9182136
ACCTAAG24000.036.81641
CTAAGAC25700.035.6514783
TTAGGTA13000.034.3365174
GTCTTAG15100.033.304391
TAGGTAT12850.033.274715
TAAGACA30300.033.1854064
ACGCAGA37450.032.9974177
GGGTAAT6250.032.3269277
AGGTATA13350.032.0284656
CCTAAGA28400.031.9399072
Produced by FastQC (version 0.11.2)