FastQCFastQC Report
Sun 14 Apr 2019
SRR6575591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464985
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA77430.5285378348583775No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC54710.37345092270569324No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC32200.2197974723290682No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG23020.15713471468991153No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC22230.15174216800854617No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC19890.13576930821817287No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT19780.1350184472878562No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA15990.10914787523421741No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT15710.10723659286613857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7300.054.7418331
GTATCAA25050.051.4240341
ATCAACG28200.043.9871753
TCAACGC28650.043.2962724
CAACGCA30450.040.736895
TATCAAC31100.039.7561042
AACGCAG31600.039.2543756
AGGTATA12500.037.9649286
TTAGGTA13300.037.0944444
TAGGTAT12950.037.008515
CTTAGGT13050.036.0048263
ACCTAAG22950.035.849131
GTCTTAG15900.034.5948941
CTAAGAC27000.032.7163853
TAAGACA30950.031.1217784
TATAGTA16350.031.036829
ACGCAGA40050.030.972247
CCTAAGA27300.030.82472
GGTATAG15450.030.7159617
TACATGG53150.030.4273172