FastQCFastQC Report
Sun 14 Apr 2019
SRR6575591_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575591_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464985
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA82610.5638965586678363No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC54220.37010617856155525No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC32670.22300569630405773No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG20270.1383631914319942No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC19720.13460888678041072No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT19300.13174196322829243No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC19200.13105936238255No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT16400.11194653870176147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7500.050.8607981
GTATCAA22600.050.427371
TCAACGC26450.041.2194024
ATCAACG26550.041.0641483
CAACGCA28550.038.68135
AGGTATA13450.038.082796
TATCAAC29400.037.562972
TTAGGTA13650.037.526084
AACGCAG29400.037.4018486
TAGGTAT13600.037.3185045
CTTAGGT13950.037.0559433
ACCTAAG23350.036.3032071
GTCTTAG16150.036.1583441
CTAAGAC26100.035.1102143
TAAGACA29300.032.8795284
GGTATAG15850.032.3163077
CCTAAGA26950.032.2591062
TCTTAGG18900.030.8318232
GTACATG54950.030.4242571
TACATGG54250.030.405122