FastQCFastQC Report
Sun 14 Apr 2019
SRR6575592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589482
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14300.2425858635208539No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7980.13537309027247652No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7280.12349825779243472No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6660.11298054902439769No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6540.11094486345639051No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6280.106534211392375No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT6030.10229319979236007No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.10110571654435589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13400.049.144391
CTAACGC450.001013733741.7762683
ATCAACG16800.038.88553
TCAACGC16850.038.491194
CAACGCA17150.037.817885
AACGCAG17400.037.544626
TACATGG27650.032.1308752
GTACATG27900.032.0331881
TATCAAC21250.031.1900482
ACGCAGA21600.030.0266937
ACATGGG29600.030.0090523
GTATAAC953.7284743E-529.7083381
TGGGTAC5150.029.2028316
TGCTATA1154.1874755E-628.6125162
CGCAGAG22950.028.4652048
GGGTACC5350.028.1111357
GGTACCT5750.026.972948
CTAGTAC1101.01776175E-425.6354393
GCAGAGT25800.025.3207939
CATGGGG17350.025.1921734
Produced by FastQC (version 0.11.2)