FastQCFastQC Report
Sun 14 Apr 2019
SRR6575594_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575594_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789939
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA63710.8065179716408482No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC35300.4468699481858726No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC28690.36319260094766814No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC23370.2958456285865111No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC21890.27711000469656516No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG15510.19634427468450097No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA13840.17520340178165658No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT13290.16824083884958208No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA12170.15406252887881217No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG11830.14975839906625701No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11780.1491254387997048No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC11330.14342879640073475No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT11120.14077036328121537No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA10880.1377321540017647No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA10560.13368120829583044No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC9990.12646546125713504No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC9930.12570590893727238No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG9920.12557931688396193No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT9920.12557931688396193No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT9900.12532613277734103No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA9870.12494635661740969No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA8880.11241374333967559No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT8660.10962871816684579No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG8620.10912234995360401No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG8250.10443844398111753No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT8020.10152682675497729No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA8000.1012736426483564No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT7900.10000772211525193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5000.059.3629531
CTAACGT250.00523669156.3965844
GTATCAA22000.050.1115881
ATCAACG24100.044.657043
TATCAAC24900.043.222282
TCAACGC25000.042.86144
AACGCAG25800.042.0788426
CAACGCA25750.041.978045
AAGGGTA4350.041.054985
GGTAATC4500.040.7308658
GTGCAAG6800.040.185011
AGGGTAA4650.038.4062736
GTACATG41800.035.955011
AGGTATA11950.035.7886286
CTTAGGT11850.035.694043
GTCTTAG13600.035.6815151
TACATGG43100.034.566352
TTAGGTA12150.034.42594
ACGCAGA31350.034.329667
TATCTCG550.002717303834.1797455
Produced by FastQC (version 0.11.2)