FastQCFastQC Report
Sun 14 Apr 2019
SRR6575604_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575604_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401070
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9630.24010771187074575No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5910.14735582317301218No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5400.13463983843219388No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5340.13314384022739173No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5100.12715984740818312No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4890.12192385369137557No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4490.11195053232602788No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4330.1079612037798888No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4080.10172787792654649No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4050.10097987882414543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12150.042.9936031
ATCAACG15550.032.9343263
CAACGCA15450.032.8433885
TCAACGC15450.032.8433884
AACGCAG15450.032.539286
ACATGGG26150.031.4426423
GCACTAC600.004164590531.3228593
TACATGG27450.031.1516952
GTACATG27500.031.1455361
ATAAGAC953.754809E-529.674293
CATTGCG650.006133286328.9386639
TATCAAC18900.027.5939482
CATGGGT5450.027.5871074
ACGCAGA17900.027.5640547
CATGGGG12350.027.0112114
TAGGACA1057.4266136E-526.8481674
ATATAGG700.00884369426.8481662
CGCAGAG18350.026.3956898
GTGTACA1901.3093086E-824.7687171
TTAGGCT950.001305646624.7285754