FastQCFastQC Report
Sun 14 Apr 2019
SRR6575607_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575607_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855860
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC103680.3630430063098331No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT70770.24780626501299083No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA51810.18141645598873896No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC50110.17546378323867418No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT44890.15718557632376937No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA44030.1541742242266778No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT41680.1459455295427647No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT40110.1404480611794696No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA39150.13708655186178592No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG37490.13127394199995798No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT34090.11936859649982842No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA33640.11779288900716423No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC33240.11639226012479602No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC31930.11180520053504024No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT30740.10763832960999488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51450.042.489461
CAACGCA51850.039.521165
ATCAACG54400.037.6686063
GTACTAG21350.035.892471
GTACATG86050.034.4740331
TACATGG87200.033.911562
ACATGGG89900.032.5701943
ACGCAGA63250.032.4722867
CATGGGG70450.030.7547284
CGCAGAG67950.030.2262298
TAGTACT36250.029.8202844
AACGCAG74150.029.156726
GTACTGT39150.028.9319176
ATGGGGG46300.028.9304945
CTGTGCG38950.028.4771489
TAGGCAT28850.028.1833255
GCAGAGT73050.028.1803159
TATCAAC77450.028.1043282
TTAGGTA5800.027.551354
CTAGTAC41650.027.3081533
Produced by FastQC (version 0.11.2)