FastQCFastQC Report
Sun 14 Apr 2019
SRR6575607_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575607_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855860
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC97870.3426988717934352No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT67930.23786179994817672No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA48830.17098177081509597No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC47100.1649240508988536No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT43570.15256350101195437No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA43330.15172312368253343No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT39140.13705153613972676No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT36500.12780738551609672No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA35310.12364051459105138No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG32410.11348595519388206No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC32400.11345093947182285No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT32250.11292570364093478No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC32050.11222538919975068No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA31060.10875883271588944No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT29300.10259606563346943No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC29080.10182571974816693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51850.044.71941
CAACGCA51250.041.634545
ATCAACG53900.039.501073
GTACTAG21950.034.353031
GTACATG87350.033.5588531
ACGCAGA64200.033.235137
TACATGG88550.033.0142332
CGCAGAG67000.031.7760548
ACATGGG92050.031.55473
AACGCAG73100.031.3114646
TATCAAC75650.030.8157062
CATGGGG70500.029.5995884
GCAGAGT72150.029.3124949
ATGGGGG46600.027.937385
CGCAATC2700.027.851953
TAGGCAT30100.027.32525
TCAACGC79600.027.2785134
CGTTAAC5750.027.0474111
CTAGGCA32100.026.5011884
TAGTACT35300.026.362244
Produced by FastQC (version 0.11.2)