FastQCFastQC Report
Sun 14 Apr 2019
SRR6575618_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575618_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608044
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC47960.2982505453830865No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT30580.19016892572591299No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC26110.16237117889808986No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA24350.15142620475559126No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA23900.14862777386688425No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG22950.1427199753240583No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT21430.13326749765553678No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT19850.12344189586852101No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC19660.12226033615995582No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA19500.12126533851063777No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT19300.12002159144899022No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC18040.11218598496061052No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC17830.1108800505458806No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA17630.10963630348423302No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT17520.10895224260032686No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA17010.10578068759312556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27400.047.284561
CAACGCA31200.039.7715455
ATCAACG32600.037.6321953
TAGTATG2500.035.717647
GTACTAG9550.035.026141
TAATAGT2700.033.0764
ACGCAGA39100.031.4915247
GTATATA1950.031.4084131
GTACATG45800.030.9626641
TACATGG44800.030.7415872
AACGCAG43600.029.8618326
TAGTACT16900.029.7591274
TATCAAC44400.029.7481522
AATAGTA2850.029.6859385
CGCAGAG41700.029.5289428
ACATGGG47350.029.086023
GGTATCA13450.028.3726581
CTAGTAC18150.027.969443
GCAGAGT44300.027.7958669
GAACCGT2203.6379788E-1227.7743456
Produced by FastQC (version 0.11.2)