FastQCFastQC Report
Sun 14 Apr 2019
SRR6575619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575563
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC47330.30040055523009873No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT29030.18425159768286004No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC27640.1754293544593266No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA25520.16197384680904542No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA24790.15734058238229764No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG24070.15277078733125873No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT21990.13956915718381302No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT20560.13049303645744412No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT19530.12395569075943014No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC19100.12122650760394856No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA18930.1201475282168977No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA18450.11710099818287177No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA18130.11506997816018781No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC17840.1132293662646305No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17670.11215038687757964No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA17640.11195997875045301No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT17560.11145222374478203No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC17320.10992895872776905No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT15970.10136059300707113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31200.048.6554261
CAACGCA33850.042.6140445
ATCAACG35400.040.749473
ACCTAAG6450.034.9755741
ACGCAGA42350.034.0610477
AACGCAG45900.033.4740376
GGTATCA13850.032.5765271
TATCAAC46600.032.4753072
CGCAGAG45050.032.1239558
GTACTAG8300.031.7097841
TACATGG48300.030.0673182
GCAGAGT48750.029.8785939
GTACATG49400.029.8734931
TAGTACT14250.029.6766224
CCTAAGA8100.029.5916042
TCAACGC52000.028.5542374
ACATGGG50200.028.548483
CTAGTAC16250.027.46993
TAGCGAG700.00884939126.8494267
CATGGGG38150.026.3576134
Produced by FastQC (version 0.11.2)