FastQCFastQC Report
Sun 14 Apr 2019
SRR6575622_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575622_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155998
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA136760.6343234084632732No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG56640.26270896355191425No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC52990.24577944877499885No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA48170.22342321282301747No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC46320.21484249985389597No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46070.21368294404725793No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC41220.19118756139847995No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC40920.18979609443051432No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA40050.18576084022341394No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC31450.14587212047506537No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG30980.14369215555858586No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT30680.14230068859062023No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT28920.13413741571188842No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA28690.13307062436978143No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA27620.1281077255173706No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG27460.12736560980112227No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT24740.1147496426249004No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT24610.11414667360544861No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC23440.10871995243038259No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA23250.10783869001733767No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA23010.10672551644296516No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA21710.10069582624844735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22550.057.7491531
GTATCAA84300.054.094931
ATCAACG101350.044.0072173
CAACGCA103100.043.4881745
GTGCAAG14600.041.538351
TCAACGC109400.041.0267874
TATCAAC110300.040.789542
AACGCAG113400.040.1184166
TAGTACT12950.038.1066674
GGTAATC15500.037.295378
AAGGGTA16100.036.781225
ACGCAGA125600.035.7351157
AGGTATA13800.035.4189536
TAGGTAT13750.035.2059445
CTTAGGT14050.034.7887233
CGCAGAG129350.034.5901158
AGGGTAA17700.033.9874156
GTACTAG8050.033.8722881
GCAGAGT133700.033.4647069
TGCAAGG17700.032.6696322
Produced by FastQC (version 0.11.2)