FastQCFastQC Report
Sun 14 Apr 2019
SRR6575624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3211478
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA116210.36185830947619757No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC74460.2318558620049709No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC68590.21357767358207033No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC44200.1376313336102567No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC42910.1336144915207266No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG37070.11542971803014065No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT33910.10559001182633043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.055.9962581
GTATCAA59100.054.013131
TCAACGC67500.046.232374
ATCAACG67750.046.1318553
CAACGCA70700.044.2727625
AACGCAG72950.043.036116
TATCAAC77700.040.6598132
ACGCAGA90400.034.7288067
TAGGTAT23750.034.2345245
TTAGGTA23950.033.3599364
GTCTTAG28900.032.6975561
AGGTATA24500.032.611046
GTACATG124700.031.6686741
CTTAGGT25550.031.6392463
TTTACGC600.004162518331.3326643
TACATGG125350.031.0543942
CGCAGAG102350.030.7658398
ACATGGG127850.030.4749053
ACCTAAG42300.029.4525281
GGTATAG27300.029.4384757
Produced by FastQC (version 0.11.2)