FastQCFastQC Report
Sun 14 Apr 2019
SRR6575624_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575624_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3211478
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA119960.37353517601552927No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC73260.22811926471238475No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC66010.20554398940301008No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC41780.1300958624035413No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC39300.12237356133219658No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT32530.10129292493985635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15900.056.005111
GTATCAA54450.052.6965751
TCAACGC62550.045.0827524
ATCAACG63150.044.655113
CAACGCA64950.043.489245
AACGCAG67450.041.947016
TATCAAC72800.038.929532
GTACATG119100.032.9135361
ACGCAGA86350.032.819717
TACATGG120200.032.41482
TTAGGTA24350.032.2332464
CTTAGGT24950.031.6469573
GTCTTAG28300.031.4657691
ACCTAAG41450.031.3723831
ACATGGG124150.030.8913353
AGGTATA24600.030.7593576
CTAAGAC45250.030.5366923
TAGGTAT25100.030.3338685
CGCAGAG94450.029.7563048
TAAGACA52500.028.736394
Produced by FastQC (version 0.11.2)