FastQCFastQC Report
Sun 14 Apr 2019
SRR6575625_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575625_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2950570
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA119000.4033119024459681No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC71410.2420210332240889No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC63630.21565324666081467No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC40610.1376344231792501No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC37640.12756857149635495No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT31000.10506444517500008No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG30180.10228532114133879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.057.5628241
GTATCAA51050.052.553831
TCAACGC57700.044.9561164
ATCAACG59000.044.0452043
CAACGCA60150.043.2812355
AACGCAG63200.041.3426136
TATCAAC68650.038.061792
GTCTTAG26200.034.3128931
ACGCAGA78650.033.2212757
AGGTATA23400.033.1366276
TAGGTAT24100.032.758035
TTAGGTA24000.032.3071174
CTTAGGT24350.032.2287183
GTACATG107450.032.1963121
TACATGG110050.031.088252
CGCAGAG86750.030.2487078
ACATGGG112750.030.0499823
GGTATAG27000.028.8924627
GCAGAGT92700.028.3071789
ACCTAAG39250.027.9409711
Produced by FastQC (version 0.11.2)