FastQCFastQC Report
Sun 14 Apr 2019
SRR6575627_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575627_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3380227
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA431431.2763344000269805No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC257210.7609252278027482No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC246410.7289747108700095No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC152480.4510939649911086No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG144000.4260068924365139No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG108940.32228604765301266No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT107990.31947558551541066No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA104490.3091212513242454No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC104050.307819563597356No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA99940.29566061687573053No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT97070.287170062838975No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT94720.2802178670249069No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC92500.27365026076651067No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA91130.2695972785259688No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG90380.26737849262786195No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA86990.25734958036841904No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT83140.24595981275813725No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA82460.2439481135438537No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT81990.2425576743810401No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT80570.23835677308062445No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT75360.22294360704177557No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA74160.21939354960480464No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC64000.18933639663845062No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA63980.18927722901450109No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG63520.18791637366366223No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA63400.18756136791996514No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC63300.18726552980021757No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT61100.18075709116577082No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG56120.1660243528023414No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA55680.16472266507545202No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG52930.15658711678239362No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC51700.1529483079094984No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG51430.1521495449861799No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG51030.1509661925071896No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC51030.1509661925071896No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA49960.1478007246258905No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA49810.14735696744626914No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA48530.14357023951350015No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA47950.14185437841896417No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC47120.13939892202505927No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC46940.1388664134095136No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG46720.13821556954606895No Hit
GACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACT46300.13697304944312913No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT46160.1365588760754825No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT45700.13519802072464365No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA45540.13472467973304753No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG45390.13428092255342616No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC45330.1341034196815776No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA45040.1332454891343096No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA44660.13212130427926883No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT44220.13081961655237948No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA43960.13005043744103575No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT43820.12963626407338916No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC43250.12794998679082795No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42280.12508035702927645No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT41190.12185572152402782No Hit
CTATGGCCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCAC40550.11996235755764333No Hit
GTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT40120.11869025364272873No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT39740.11756606878768792No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT39610.11718147923201608No Hit
CTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAG39580.1170927277960918No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA39090.11564312100932866No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT38530.11398642753874223No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC38480.11383850847886844No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCACCTCATTAACGAAA38460.1137793408549189No Hit
CCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCAGAC37570.11114638158916545No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA37560.1111167977771907No Hit
ATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGAC36680.10851342232341202No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA36110.1068271450408508No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA35810.10593963068160808No Hit
ATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACC35450.10487461345051678No Hit
GAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAG35440.10484502963854203No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG34010.10061454452615164No Hit
ATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAACTTTAG33980.10052579309022737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21250.067.200041
GTGCAAG23500.049.9364131
AAGGGTA24650.045.569755
GTATCAA105650.045.455931
ATCAACG102800.044.9887663
TATCAAC106750.043.808392
TCAACGC105600.043.7952354
CAACGCA105500.043.7921945
GGTAATC26050.042.937748
AACGCAG110150.042.114186
AGGGTAA27050.041.7003446
TGCAAGG27650.039.946142
GTAATCA32050.036.659119
ACGCAGA131650.034.9125487
GTACTAG3550.034.5168271
TAGGTAT69350.034.0214585
TTAGGTA69600.033.8317264
CGCAGAG136700.033.657688
GCAAGGG32850.033.479783
AGGTATA71000.033.3632136
Produced by FastQC (version 0.11.2)