FastQCFastQC Report
Sun 14 Apr 2019
SRR6575628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3258573
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA434921.3346946654256326No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC252600.7751859479594289No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC250350.7682810850025457No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG163480.5016919983072344No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC162790.4995745070004569No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA119440.36654081403117256No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT111900.34340185105566146No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG109850.33711075369494564No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA107660.33039002041691257No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC104830.32170523723114386No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA103490.3175930077368222No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC99500.3053483840932826No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT95260.29233655345453363No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT93660.2874264286851944No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT92730.28457241866301597No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT84710.25996041825670313No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA84030.257873615229734No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA83920.2575360441518419No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT80420.24679514621891238No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT78820.24188502144957316No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA66880.20524321535837928No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA65830.20202094597850043No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA64780.19879867659862155No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT64320.19738701572743653No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG63140.19376579871004884No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG62970.19324409795330655No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC60850.1867381826339321No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG60640.18609372875795632No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA57350.17599728470100257No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG56470.17329671607786598No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA55500.17031995293645408No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC55440.17013582325760387No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC52950.1624944415853197No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG51700.15865840660927344No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT51320.1574922519765554No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG49980.15338002248223379No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG49640.1523366209687492No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA49360.15147734913411484No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC48960.15024981794178005No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA48630.14923710470810383No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG48460.14871540395136154No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC48220.14797888523596064No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC47730.1464751595253505No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA47570.14598414704841658No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA47540.1458920822089915No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT46920.14398940886087253No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC46910.14395872058106418No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT46090.1414422816367778No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA45660.14012268560501792No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT44840.13760624666073154No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT44480.13650146858763024No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC44240.13576494987222937No Hit
GACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACT43930.13481361319816987No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA43180.13251199221254212No Hit
CTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAG42180.1294431642317051No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA41910.12861458067687911No Hit
GTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT40790.12517749333834166No Hit
ATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGGAA40210.12339757310945619No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT39790.12210866535750464No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA39270.12051287480746939No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38540.11827263038145838No Hit
CTATGGCCGAGAACCAGATTTGAGTTCAGACATCAAGGAAAGGTTTGCAC38390.11781230618433283No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCACCTCATTAACGAAA38020.11667683983142313No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT37980.11655408671218967No Hit
ATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAAGACAGAC37810.11603238595544736No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA37540.11520380240062136No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC37340.11459003680445398No Hit
CCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTTCAGAC37270.11437521884579539No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT36890.11320906421307732No Hit
GGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGA36860.11311699937365222No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG35710.10958784719568965No Hit
CAGTGAGACAGGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTG35540.10906614643894737No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT35540.10906614643894737No Hit
ATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTG35500.10894339331971388No Hit
GAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAG34890.1070714082514033No Hit
ATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAACTTTAG34740.10661108405427774No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA34570.10608938329753545No Hit
ATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACC34330.10535286458213458No Hit
ATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGC33650.1032660615551654No Hit
CTCTTACAGTATGGAATTTAAGAACTAAGGAATTCTCCAAGACATGGATT33550.1029591787570817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20500.069.938821
GTGCAAG29200.046.3641131
ATCAACG101900.044.0828253
AAGGGTA31550.043.3390435
TATCAAC104100.043.2155652
TCAACGC104350.043.0478174
CAACGCA104950.042.846485
GTATCAA109050.042.762031
AACGCAG108250.041.670536
GGTAATC32400.041.3312848
AGGGTAA33250.040.699266
TGCAAGG34500.038.6970862
GTAATCA38000.036.599959
TAGGTAT73050.036.0851365
TTAGGTA73450.035.952594
CTTAGGT73900.035.60653
AGGTATA74100.035.5103956
GTCTTAG77450.035.0208551
GTACTAG2850.034.6375161
ACGCAGA130200.034.608817
Produced by FastQC (version 0.11.2)