FastQCFastQC Report
Sun 14 Apr 2019
SRR6575631_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575631_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3315474
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC80950.24415815053895762No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA60000.18096959891707792No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT57000.17192111897122403No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT56360.1699907765827752No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT50410.15204462469016497No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT49870.15041589829991125No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG47550.14341840714178425No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA46570.140462570359472No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA45210.13636059278401821No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA44020.13277136240549617No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC43330.13069021201794975No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC41830.1261659720450228No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC38400.11582054330692988No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA38330.11560941210819328No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG36870.11120581853454438No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT36330.10957709214429068No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT35630.10746578015692478No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA35570.10728481055800769No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA34920.10532430656973935No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT34670.10457026657425153No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC33750.10179539939085633No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT33490.101011197795549No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG33340.1005587737982563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49350.050.1048771
ATCAACG58050.040.1571273
CAACGCA58500.039.848225
GTACTAG15650.038.448291
AACGCAG75600.033.321626
ACGCAGA72100.032.136227
TCAACGC76800.030.781514
GTACATG101550.030.04321
TACATGG102550.029.70442
CGCAGAG77850.029.702278
ACATGGG105050.029.0356673
TAGGCAT24500.028.5827085
TAGTACT27000.028.0249774
CTAACGC7700.027.4666043
GCAGAGT84950.027.3857739
CTAGTAC28400.027.1399273
CTAGGCA26900.026.7314384
TATCAAC92950.026.6022132
CCTAGTA29100.026.493072
TCTAACG8000.025.8549732
Produced by FastQC (version 0.11.2)