FastQCFastQC Report
Sun 14 Apr 2019
SRR6575631_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575631_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3315474
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC77910.23498902419382567No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA57740.17415307735786797No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT56800.17131788697483377No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT55160.16637138460443365No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT50740.1530399574842089No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG49720.14996347430261855No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT46410.1399799847623598No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA41910.12640726484357892No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA40900.12336094326180812No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA39840.12016381368093974No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC39310.11856524889050554No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC39300.11853508729068604No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC38050.11476488731324691No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA36820.11105501053544681No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA36330.10957709214429068No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT34500.1040575193773198No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG33930.10233830818760757No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC33910.10227798498796854No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA33430.10083022819663193No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG33230.10022699620024166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50900.046.283961
ATCAACG57350.039.503543
CAACGCA58300.039.1811335
GTACTAG15650.037.3323751
AACGCAG74950.032.0448726
GTACATG102550.031.9320951
TACATGG102100.031.8108062
ACGCAGA73650.030.8846457
ACATGGG106000.030.41873
TAGTACT26600.029.6854384
CGCAGAG78250.029.1291248
TCAACGC79900.028.8835494
GTATATA4600.028.6799551
CTAGTAC27850.028.3534873
CATGGGG77950.027.7368774
CCTAGTA29200.026.8816552
TAGGCAT24350.026.8302735
GTACTGT30200.026.7689156
GCAGAGT85950.026.4648519
TATCAAC90650.026.3920042
Produced by FastQC (version 0.11.2)