FastQCFastQC Report
Sun 14 Apr 2019
SRR6575637_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575637_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328650
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6160.18743343982960597No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4190.12749125209189108No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3720.11319032405294387No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3590.109234748212384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10150.037.981141
ACATGGG21800.034.9258163
GTACATG22300.034.785581
TACATGG22300.034.785582
GGTACCT2200.032.0447278
AACGCAG12450.030.9551226
CATGGGA11200.030.21364
TCAACGC12850.029.6257864
CATGGGG6850.029.5029814
CAACGCA12950.029.3970175
ATCAACG13150.028.9499153
GGGTACC2450.026.8565357
ATGGGTA3500.026.8565335
ACAGTAC1606.886148E-826.4369018
CTAGTAC909.504432E-426.110523
CATGGGT5250.025.9613174
ATACCGT1101.01632686E-425.6357826
TACCGTC1101.01632686E-425.6357827
ACGCAGA15000.024.7527717
GTTATAT1151.3716627E-424.5286461
Produced by FastQC (version 0.11.2)