FastQCFastQC Report
Sun 14 Apr 2019
SRR6575637_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575637_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328650
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5980.18195648866575384No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.13570667883766926No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3910.11897155028145444No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3850.11714589989350374No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3360.10223642172523961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10950.049.5306931
ATCAACG13100.041.6218223
AACGCAG13400.039.988446
TCAACGC13550.039.892664
CAACGCA13850.038.689185
GTAATAG906.0083585E-736.6814271
ACGCAGA15600.034.645087
GTATAAC550.002668826834.299511
CGCAGAG15850.034.098638
TATCAAC16400.032.960092
TACATGG20600.032.8571362
GTACATG20850.032.5722051
ACATGGG20750.032.166563
GAGTATC600.00408733531.4412211
TGTACCG600.00415498631.335975
GGGTACC2800.030.212237
GCAGAGT17700.030.003619
ATCCTAC804.7754432E-429.3730037
CATGGGT6300.027.6054974
ATAATCG700.00882993826.8553167
Produced by FastQC (version 0.11.2)