FastQCFastQC Report
Sun 14 Apr 2019
SRR6575648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300499
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5870.19534174822545164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.13444304307169078No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3990.13277914402377378No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3810.1267891074512727No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3070.10216340154210164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8600.040.437421
GTACATG18300.039.547571
TACATGG18300.038.777162
CCCTATA500.001700943937.5958751
TACGACG500.001702335437.5896155
ACATGGG18500.037.5896153
CGACGGT550.002717507534.172387
ACGACGG550.002717507534.172386
CATGGGA9100.033.0458154
AACGCAG10650.032.648266
CAACGCA11000.031.609455
TCAACGC11100.031.324684
ATCAACG11200.031.0449963
CATGGGT4250.029.8505764
GGGTACC2051.8189894E-1229.7966487
CATGGGG6850.028.809564
CGCAGAG12700.027.748248
ATGGGTA2550.027.6394215
TATCAAC13100.027.6229232
ACGCAGA12600.027.595557