FastQCFastQC Report
Sun 14 Apr 2019
SRR6575648_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575648_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300499
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6000.19966788575003577No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4470.14875257488377666No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3970.132113584404607No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3730.12412686897460556No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3310.11015011697210306No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3260.1084862179241861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT5150.038.32864
GTATCAA10550.036.571991
GGGTACC2700.036.5480467
ACATGGG19200.035.0038453
TACATGG19250.034.9129262
GTACATG19500.034.7468261
GGTACCT3000.032.9151548
ATGGGTA3050.032.3593985
AACGCAG12350.031.5858126
ATCAACG12450.030.5771243
CAACGCA12900.030.2391345
TCAACGC12850.029.6253054
ATGGGTG1952.0008883E-1128.9219535
TGCCGTC650.00615320428.917147
TGGGTAC3300.028.4837386
CATGGGG5800.028.3609624
CTTATAG1005.3120442E-528.1989043
GTACCTG3600.027.4292959
ACGCAGA14400.027.0847157
GGTTAGC700.0088060226.8695138
Produced by FastQC (version 0.11.2)