FastQCFastQC Report
Sun 14 Apr 2019
SRR6575655_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575655_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3639561
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA326160.8961520359186176No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC192560.529074797757202No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC148020.4066974011426103No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG105050.28863371159323886No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC105010.28852380822851986No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC94850.2606083535898972No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT85880.23596252405166446No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT80810.2220322725735329No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA73370.2015902467358014No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC69060.18974815918733057No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC66910.18384085333368502No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT66700.18326386066891034No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT66610.18301657809829264No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA66360.18232968206879896No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA64560.17738403065644456No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG59590.16372853759011047No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA59330.16301416571943705No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT57470.15790365926000416No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG55070.15130945737686496No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT52800.14507244142906245No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG52050.14301175334058147No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA48510.13328530556295112No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT48310.1327357887393562No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA47670.1309773349038524No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA44740.12292691343818664No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT44550.12240487245577146No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG43230.1187780614200449No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG43140.11853077884942717No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA41470.11394231337240948No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT40540.11138706014269303No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC38880.10682607050685508No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA38080.10462800321247535No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC37950.10427081727713865No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG37910.10416091391241965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36950.10152323315916398No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA36870.10130342642972599No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC36480.10023186862371587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22900.059.8822061
GTATCAA91800.049.1257861
ATCAACG96750.045.4748953
TCAACGC99600.044.220254
TATCAAC102250.043.3965842
CAACGCA101400.043.388325
AAGGGTA16400.043.2780845
AACGCAG105500.041.8357966
AGGGTAA18000.038.386616
GGTAATC18100.037.1316838
GTGCAAG22800.036.9963151
ACGCAGA125650.035.01027
TAGGTAT58750.034.242925
TTAGGTA58500.034.0683334
CGCAGAG129600.033.9073458
GTCTTAG64700.033.7222821
AGGTATA59400.033.4725536
CTTAGGT59800.033.3281753
GTACATG176050.032.3348771
GCAGAGT138550.031.818349
Produced by FastQC (version 0.11.2)