FastQCFastQC Report
Sun 14 Apr 2019
SRR6575658_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575658_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402219
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA254991.0614769094741154No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC187830.7819020663811251No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC149140.6208426459036416No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC98250.4089968483306476No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG71810.2989319458384102No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA59580.2480206842090584No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG53860.22420936642329448No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC53740.2237098282879288No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA53450.22250261112746172No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG52150.2170909479943336No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC51360.21380232193650953No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT48970.20385318740714317No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT47930.19952385690064062No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT46120.1919891566922083No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT46090.1918642721583669No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG45790.19061542681995272No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA44830.18661912173702733No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA44810.18653586538113304No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT44780.1864109808472916No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG44640.18582818635603165No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA44450.185037250975036No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT44280.18432957194993463No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT43720.1819983939848948No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC40930.17038413233764282No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT39490.1643896747132547No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA39380.16393176475583618No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT38880.16185035585847918No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA37590.15648032090329814No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT37250.1550649628530954No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC36840.15335820755726268No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA36280.15102702959222286No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG36220.15077726052454002No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA35430.14748863446671598No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG35290.14690583997545603No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA34320.14286790671458346No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA33330.13874671709781664No Hit
CTATTATGCTGGCCACTGACAAAAGAGAAAAGATAGAAGAACATGGCAGC33250.13841369167423953No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA33190.1381639226065567No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT33010.13741461540350816No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC32920.1370399618019839No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC32530.13541646286204548No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT31820.13246086222779854No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA30730.12792339083156032No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA30500.1269659427387761No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA30220.1258003537562562No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT29200.12155427960564794No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC29160.12138776689385938No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG28170.11726657727709257No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA27930.1162675010063612No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA26980.11231282410138292No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG26980.11231282410138292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26970.11227119592343579No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG26960.11222956774548866No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT26640.11089746605118017No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA26130.10877442897587605No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC25480.10606859740931197No Hit
CCTCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGT24850.10344602219864217No Hit
CTGTAAGAGATGAAGAGTGCTCGGAATTATCTATGGTTGCTGACAAAACA24720.10290485588532935No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC24610.10244694592791082No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA24270.10103158787770808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12450.064.2580641
GTATCAA52300.053.628131
GTGCAAG17000.045.95231
AAGGGTA13200.044.501855
TCAACGC63600.043.3733524
GGTAATC13450.043.351458
ATCAACG63150.043.2359283
CAACGCA65000.042.3668565
TATCAAC65150.042.12682
AACGCAG66850.041.4035726
AGGGTAA15300.038.699296
GTACATG107100.036.2503781
CAAGGGT17550.035.613654
TGCAAGG16800.035.526672
ACATGGG109800.035.0100673
ACGCAGA78700.034.9895027
ATGGGGG35000.034.9097945
TACATGG111600.034.699492
CGCAGAG81250.033.971838
CATGGGG71950.033.637094
Produced by FastQC (version 0.11.2)