FastQCFastQC Report
Sun 14 Apr 2019
SRR6575670_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575670_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390807
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6890.17630185743858223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5050.12921979391361976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11850.039.3460661
CGATACC608.820463E-539.161334
GTTAGTC500.001701790337.5948833
CCGATAC550.002716637434.1771623
GTACATG24400.033.584891
TACCGTG702.1841197E-433.566867
TACATGG24100.033.356812
ACATGGG24850.032.526763
CGTACGG300.00416294731.32906584-85
ATCAACG15400.029.5998653
CATGGGG9550.029.5247764
AACGCAG15350.028.777846
TCAACGC15850.028.7594914
CAACGCA16050.027.8155275
TATCAAC16750.027.7859922
ACCGTGC856.787951E-427.6609928
CATGGGA12800.027.1681774
CATGGGT4750.026.7121524
ATACCGT1258.0146765E-626.3164186
GTAACCC909.5139554E-426.1075573
Produced by FastQC (version 0.11.2)