FastQCFastQC Report
Sun 14 Apr 2019
SRR6575675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259227
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA74560.3300243844465386No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC46760.20697344711266288No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC45790.2026799431841068No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC32160.14234957354882888No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA31840.14093315988167635No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC30290.13407240618140628No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT29540.13075268664901757No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT29330.1298231651799487No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT27700.12260830806289054No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA27310.12088205390604839No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG25730.11388851142448281No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA25690.11371145971608874No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA25230.11167536506955697No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC24800.10977205920432076No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT24680.10924090407913858No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG24480.10835564553716825No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG24060.10649660259903054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8850.050.9968261
GTATCAA35000.049.4304921
ATCAACG38100.044.6553763
TCAACGC38550.044.0112154
CAACGCA40000.042.4158135
AACGCAG41550.041.059746
GTACATG75600.036.2544981
TACATGG75800.036.2208632
ACATGGG78450.034.5679053
AGGTATA17750.033.0972756
CTTAGGT18150.032.8864753
TAGGTAT18100.031.9379565
GTCTTAG20500.031.8769021
GGTAATC6750.031.3320878
TATCAAC55650.031.0038812
ACGCAGA55300.030.8504927
TTAGGTA19200.030.3529594
CATGGGG53100.029.4734044
GGTATAG20450.029.4169247
CGCAGAG59000.029.0751118
Produced by FastQC (version 0.11.2)