FastQCFastQC Report
Sun 14 Apr 2019
SRR6575675_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575675_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2259227
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA72160.31940128194289463No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC45890.20312257245509194No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC43310.1917027372636747No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC31780.14066758231908524No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA29470.13044284615932794No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT27850.12327225196936828No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC26190.11592460607101455No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT25840.11437540362256647No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT25260.11180815385085252No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG24770.10963927042302524No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT24580.10879827480815342No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA23690.10485887429638545No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA23570.10432771917120326No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG22900.10136210305560264No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC22690.10043258158653381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7950.057.506911
GTATCAA33650.052.524461
TCAACGC39400.043.897984
ATCAACG39800.043.3406263
CAACGCA40700.042.495845
AACGCAG42050.041.243296
GTACATG71700.034.0506971
TACATGG72550.033.36432
ACATGGG74700.032.341093
TATCAAC54150.032.2891542
ACGCAGA54400.031.705967
CTTAGGT16200.031.62453
TTAGGTA16650.031.05074
AGGTATA16500.031.0481346
GTCTTAG19000.031.0078621
TAGGTAT16700.030.9577335
CGCAGAG57550.029.9705338
GGTATAG17850.028.4353967
ACCTAAG26800.028.138481
CTAAGAC28900.027.9732423
Produced by FastQC (version 0.11.2)