FastQCFastQC Report
Sun 14 Apr 2019
SRR6575676_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575676_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2088200
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA72330.34637486830763337No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC44290.21209654247677426No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC41490.19868786514701656No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC30220.14471793889474188No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA27250.1304951632985346No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT25910.12407815343357916No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC25420.12173163490087155No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT24830.11890623503495834No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT23220.11119624557034767No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT22620.10832295757111388No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG22580.10813140503783163No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA21980.10525811703859785No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA21890.10482712383871276No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG21430.10262426970596686No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC21080.10094818503974715No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG20950.10032563930657984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30850.051.7103041
GGTATCA8050.049.1039051
TCAACGC37400.041.7153134
ATCAACG38100.040.948893
CAACGCA39050.040.193385
AACGCAG40400.038.733966
TAGACCG702.1876454E-433.566115
GTACATG67950.032.8262631
TACATGG68450.032.1300852
ACATGGG69850.031.821733
TATCAAC50150.031.2978842
ACGCAGA51950.030.1222727
CGTATAG650.006160069328.9184933
CGCAGAG54400.028.6127628
CATGGGG44800.028.5311934
GTCTTAG18700.026.926181
GCAGAGT59650.026.3309639
ACCTAAG25950.025.7503761
GGTAATC5700.025.5751138
AGGTATA15700.025.4418286
Produced by FastQC (version 0.11.2)