FastQCFastQC Report
Sun 14 Apr 2019
SRR6575677_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575677_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047357
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA108210.3550945950868244No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC65500.214940356512217No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC61350.20132199804617576No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC49030.160893521828916No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA45010.1477017625437387No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC41760.13703678302214017No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT40090.13155662431411874No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT38010.12473103742029568No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT37270.1223027036215317No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA36840.12089164479252021No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG35830.11757729731042342No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA35780.11741322070239883No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT35460.11636313041104145No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA35030.11495207158202993No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC34690.11383635064746271No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG32110.1053699976733937No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG31970.10491058317092485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13150.057.5572281
GTATCAA48700.052.223751
TCAACGC57500.043.074614
ATCAACG57700.042.76243
CAACGCA60200.041.220765
AACGCAG63250.039.5302666
TACATGG102400.033.513722
GTACATG103500.033.3392221
TATCAAC76100.032.864482
ACATGGG107950.031.5206053
ACGCAGA80300.031.0197937
CGCAGAG84000.029.6534488
ACCTAAG39650.029.0484281
TAGGTAT20700.028.3803835
CATGGGG68200.028.322794
GCAGAGT88200.028.2946629
CTAAGAC44350.028.2941253
AGGTATA20650.027.5387296
GTCTTAG25050.027.2119751
CCTAAGA44100.026.9700472
Produced by FastQC (version 0.11.2)