FastQCFastQC Report
Sun 14 Apr 2019
SRR6575678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2816542
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA108150.3839814921985896No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC66110.234720447981958No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC58410.20738195986425909No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC45840.16275276562536614No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC40740.14464545531364348No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA40500.14379334659309181No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT35740.12689319030215065No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT34980.12419484602040375No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT34930.12401732337028883No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA34840.12369778260008193No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA33960.12057338395805922No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG33210.11791054420633529No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT33030.11727146266592155No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG31930.11336596436339313No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA31490.11180376504238175No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG31230.11088064726178413No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC30650.10882138452045097No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG28810.1022885509962216No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT28540.10132992868560099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12000.054.0517851
GTATCAA48000.051.1141851
TCAACGC54600.043.545074
ATCAACG55200.043.1568763
CAACGCA56150.042.3422745
AACGCAG58200.040.8508346
GTACATG94300.033.394531
TACATGG93950.033.168742
ACGCAGA73900.032.1721087
ACATGGG97200.031.9050033
TATCAAC76600.031.4161832
ACCTAAG38250.030.2284531
CGCAGAG78900.030.1928678
GTCTTAG22950.030.105571
TAGGTAT19250.030.0225645
CTTAGGT19250.029.7790093
CTAAGAC42050.029.0528343
ACGTACC650.00616375228.9153043
TTAGGTA20600.028.5117514
AGGTATA20400.028.3301166
Produced by FastQC (version 0.11.2)