FastQCFastQC Report
Sun 14 Apr 2019
SRR6575678_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575678_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2816542
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA81970.2910306325984132No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC51440.18263530243823808No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC44900.1594153398032055No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39850.14148555214159775No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC34070.12096393378831205No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA30810.10938945700081873No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT28180.10005176560477351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13600.050.183681
GTATCAA43300.049.7864341
ATCAACG50900.041.3610423
TCAACGC52400.041.3428964
CAACGCA53700.040.4295545
AACGCAG54850.039.4105456
TATCAAC64300.033.036162
GTACATG88500.032.442921
TACATGG88500.031.8086782
ACATGGG92200.030.988743
ACGCAGA69400.030.877117
CGCAGAG73850.029.290318
AGGTATA22000.028.8364856
TAGGTAT21850.028.604315
ACCTAAG37100.027.9114381
CTAAGAC40700.027.5952763
CTTAGGT22200.027.5183013
TTAGGTA22700.027.119194
GCAGAGT79050.027.0066439
TAAGACA47250.026.952484
Produced by FastQC (version 0.11.2)