FastQCFastQC Report
Sun 14 Apr 2019
SRR6575681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266730
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6360.23844336969969632No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4690.1758332396055937No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4140.155213136879991No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3670.1375923218235669No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3460.12971919169197316No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3360.12597008210549995No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3100.11622239718066958No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2900.10872417800772316No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2840.10647471225583925No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG2830.10609980129719192No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2810.10534997937989728No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2790.10460015746260264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.10460015746260264No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2780.10422524650395532No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2750.10310051362801334No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2680.1004761369174821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC156.876634E-494.0677261
GTATCAA8400.038.634961
ACATGGG18550.038.2576183
GTACATG19000.037.6270941
CGACGGA250.001701459437.59887790-91
CTACACC651.41083E-436.1527634
TGGGTAT1055.088259E-835.8084536
TACATGG20350.035.5931972
CATGGGT4650.035.3752864
ATGGGTA3100.034.8699265
ATGGGAC1353.43789E-1034.8137745
CATGGGG6650.034.6305434
GGGTATG753.2694262E-431.3323967
AGGCTTA1901.4551915E-1129.6833216
GTTCCAT650.006124217528.9439161
CTCTAGG650.006146711828.922217
AACGCAG11150.028.6628196
CAACGCA11200.028.534865
TCAACGC11050.028.071564
CATGGGA8750.027.9305934
Produced by FastQC (version 0.11.2)