FastQCFastQC Report
Sun 14 Apr 2019
SRR6575681_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575681_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266730
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6880.25793873954935703No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5000.18745547932366063No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4780.17920743823341956No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4320.16196153413564277No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4110.15408840400404905No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.14021669853409816No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3650.13684249990627226No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3470.13009410265062046No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3310.12409552731226334No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3200.1199715067671428No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3160.11847186293255352No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3030.11359802047013834No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2990.11209837663554907No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2990.11209837663554907No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG2950.11059873280095976No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2940.11022382184231246No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT2750.10310051362801334No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2740.10272560266936603No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2730.1023506917107187No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG2730.1023506917107187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGTA352.9341938E-453.7113955
GGGTACC2100.046.9974677
GGTACCT2300.042.9107328
ATGGGTA2800.041.962035
GTATCAA10150.040.4351581
ATAGACG704.623558E-640.2835468
CATGGGT4050.038.294234
GTACCTG2650.037.243289
GTACATG18150.036.9077571
ACATGGG18300.036.2111663
TACATGG19000.034.8770682
GTTAGTA550.002664453834.308621
GTCTTAA550.002664453834.308621
CAATGCA550.002714002334.1799779
ACAATGC702.181213E-433.5696228
ATCAACG11750.032.7982333
AACGCAG11350.032.7118956
CAACGCA11450.032.0157435
TCAACGC11750.031.5982974
TAGACGA902.587471E-531.3316489
Produced by FastQC (version 0.11.2)