FastQCFastQC Report
Sun 14 Apr 2019
SRR6575684_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575684_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402032
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA28720.20484553847558404No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA19250.13730071781528524No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC18250.13016821299371198No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17880.12752918620972986No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17510.12489015942574778No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15220.108556723384345No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14830.10577504650393145No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA14820.1057037214557157No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG14450.10306469467173358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14170.10106759332169307No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14140.10085361817704588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6850.056.27551
GTATCAA22450.050.884561
TCAACGC27350.040.382964
ATCAACG27700.039.7030373
CAACGCA28250.039.429165
AACGCAG29750.037.9150736
GTACATG46200.036.3263971
TACATGG46150.036.0601582
ACATGGG47000.035.2991683
GTCTTAG8600.032.251481
TATCAAC34600.031.6574462
ACGCAGA36900.030.5683887
CTTAGGT7150.030.2370513
TAGGTAT7250.029.8199885
CATGGGG28750.029.261924
CGCAGAG39400.028.3901968
TTAGGTA7300.028.32814
AGGTATA7750.026.0768056
GGTATAG7800.025.9096477
ACCTAAG11650.025.825581