FastQCFastQC Report
Sun 14 Apr 2019
SRR6575685_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575685_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305363
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA30010.22989773725775897No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC17470.1338325048281589No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17250.13214714987325366No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17210.1318407216996345No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15420.11812806093017805No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13310.10196397477176845No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13280.10173415364155411No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13160.1008148691206967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7150.057.256671
GTATCAA20450.051.082551
TCAACGC24650.041.9439434
ATCAACG24700.041.8590353
CAACGCA25700.040.230285
AACGCAG26400.039.340086
GTACATG41550.032.6162761
TACATGG41650.032.6109162
CTTAGGT6100.032.3580973
TATCAAC34650.031.1964192
ACGCAGA33150.031.1866997
TAGGTAT6250.030.8295615
ACATGGG43900.030.6171673
GTCTTAG8250.029.6593931
TTAGGTA6650.028.9751534
CGCAGAG36300.028.4989728
AGGTATA6800.027.6438146
GCAGAGT38250.027.0460859
CTAGACG700.0088398626.855023
ACGCGTT700.0088398626.855025
Produced by FastQC (version 0.11.2)