FastQCFastQC Report
Sun 14 Apr 2019
SRR6575688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312641
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA159200.4805833170572966No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC90290.2725619830220057No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC83830.2530609263122687No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC61810.18658828409115266No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG57100.17237002138173138No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC50760.15323121340344456No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46100.1391638876654609No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA43420.1310736659964059No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA42840.12932279712772982No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT39530.11933076961856114No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA37580.11344422773249502No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG37180.11223673196099426No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC37070.11190467062383155No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT36320.10964061605226766No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC36160.10915761774366736No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT34950.1055049430348776No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT34770.10496156993770227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23400.062.28041
GTATCAA92350.052.07681
ATCAACG112250.041.660483
TCAACGC113400.041.362334
CAACGCA114100.041.1085745
TATCAAC115200.041.053582
AACGCAG119000.039.4553646
GGTAATC16300.035.4654168
AAGGGTA17750.033.3625875
ACGCAGA142200.032.95217
GTACATG181400.032.9133451
TACATGG186150.032.0987432
CGCAGAG145700.032.0960128
ACATGGG185350.031.8735353
AGGGTAA18300.031.332596
GTGCAAG22700.030.857871
ACCTAAG57500.030.41451
GCAGAGT154550.030.379749
CTAAGAC63550.029.1385693
TTAGGTA30150.028.9943354
Produced by FastQC (version 0.11.2)